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目的 观察实验性偏头痛大鼠脑干组织 (Gsa和Gia)G蛋白两个亚基含量的变化 ,探讨其在偏头痛发作时的作用。方法 皮下注射硝酸甘油 ( 10mg/Kg)建立实验性偏头痛大鼠模型 ,将动物随机分成正常对照、生理盐水对照和模型组 ,运用免疫印迹法 (Westernblot)检测脑干组织Gia和Gsa含量。结果 皮下注射硝酸甘油 4h后脑干组织Gsa蛋白含量明显升高 (P <0 0 1) ,Gia蛋白含量明显降低 (P <0 0 1) ,Gsa/Gia蛋白比值升高。结论 偏头痛发作可能与大鼠脑干组织G蛋白信号传导系统功能障碍有关。
Objective To observe the changes of G subunits of G protein in brain stem cells (Gsa and Gia) of experimental migraine rats and to explore its role in the onset of migraine. Methods Experimental migraine rat models were established by subcutaneous injection of nitroglycerin (10 mg / kg). The animals were randomly divided into normal control group, saline control group and model group. Western blotting was used to detect the content of Gia and Gsa in brain stem tissues. Results The content of Gsa protein in brainstem tissue after subcutaneous injection of nitroglycerin for 4 h was significantly increased (P <0.01), the content of Gia protein was significantly decreased (P <0.01), and the ratio of Gsa / Gia protein increased. Conclusions Migraine attacks may be related to dysfunction of G protein signaling system in rat brainstem tissue.