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目的探讨干扰素-γ(IFN-γ)对人肺腺癌细胞增殖和B7分子表达的影响。方法体外培养人肺腺癌SPC-A-1细胞,用不同浓度IFN-γ干预后,通过MTT比色法观察IFN-γ对SPC-A-1细胞增殖的影响,流式细胞仪(FCM)检测SPC-A-1细胞的凋亡,RT-PCR检测SPC-A-1细胞B7-1和B7-2 mRNA的表达。结果MTT结果示不同浓度的IFN-γ均可直接抑制SPC-A-1细胞的增殖;FCM检测结果示给药组的细胞凋亡率显著高于空白对照组,但不同浓度给药组之间的凋亡率无显著性差异;RT-PCR示干预后SPC-A-1细胞的B7-1和B7-2基因表达较空白对照组明显增加,但不同浓度组之间B7-1和B7-2基因表达的增加无显著差异。结论IFN-γ能直接抑制SPC-A-1细胞增殖,促进SPC-A-1细胞凋亡,并能显著上调B7-1和B7-2基因在SPC-A-1细胞表达。IFN-γ增加SPC-A-1细胞B7-1和B7-2 mRNA表达,可能是促进肺腺癌细胞凋亡的机制之一。
Objective To investigate the effects of interferon-γ (IFN-γ) on the proliferation and B7 expression in human lung adenocarcinoma cells. Methods The human lung adenocarcinoma SPC-A-1 cells were cultured in vitro. The effects of IFN-γ on the proliferation of SPC-A-1 cells were observed by MTT colorimetric assay. Flow cytometry (FCM) The apoptosis of SPC-A-1 cells was detected, and the expression of B7-1 and B7-2 mRNA in SPC-A-1 cells was detected by RT-PCR. Results The results of MTT showed that different concentrations of IFN-γ could directly inhibit the proliferation of SPC-A-1 cells. The results of FCM showed that the apoptosis rate of the drug-treated group was significantly higher than that of the blank control group. However, The apoptosis rates of B7-1 and B7-2 in SPC-A-1 cells were significantly increased compared with the control group after RT-PCR. However, the B7-1 and B7- 2 gene expression increased no significant difference. Conclusion IFN-γ can directly inhibit the proliferation of SPC-A-1 cells, promote the apoptosis of SPC-A-1 cells and up-regulate the expression of B7-1 and B7-2 genes in SPC-A-1 cells. IFN-γ increased B7-1 and B7-2 mRNA expression in SPC-A-1 cells may be one of the mechanisms of promoting apoptosis of lung adenocarcinoma cells.