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目的 在酵母细胞中表达IL-18。方法 用Sac I 将pPIC9K-IL18线性化后,电转化毕氏酵母GS115,筛选后得到His+Mut+转化子,在BMGY和BMMY培养基中进行诱导表达,表达产物进行SDS-PAGE和Western blot 分析。结果 SDS-PAGE和Western blot印迹杂交结果证实表达蛋白的相对分子量为21 000,与预期的分子量一致。结论 在毕氏酵母中成功的表达了IL-18。
Purpose To express IL-18 in yeast cells. Methods After pPIC9K-IL18 was linearized with Sac I, Pichia pastoris GS115 was transformed into E. coli by electroporation. The His + Mut + transformants were obtained after transformation and expressed in BMGY and BMMY medium. The expressed products were analyzed by SDS-PAGE and Western blot. Results The SDS-PAGE and Western blot hybridization results confirmed that the relative molecular weight of the expressed protein was 21 000, consistent with the expected molecular weight. Conclusion IL-18 was successfully expressed in Pichia pastoris.