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目的探讨脐血间充质干细胞(UCB-MSCs)移植对大脑中动脉闭塞(MCAO)模型大鼠神经功能恢复的治疗效果及其作用机制。方法 MCAO模型大鼠随机分为实验组(n=20)和对照组(n=10),实验组大鼠经尾静脉输入经BrdU(5-溴脱氧核苷尿嘧啶)标记的UCB-MSCs,对照组尾静脉注射等量PBS,分别对大鼠神经功能缺损情况进行评分,并测定大鼠梗死灶体积,计数脑组织中BrdU阳性细胞、BrdU/NSE(神经元特异性烯醇化酶)、BrdU/GFAP(胶质纤维酸性蛋白)双阳性细胞。结果移植UCB-MSCs可有效降低MCAO大鼠神经功能缺损评分,减少大鼠脑梗死灶体积,并且实验组大鼠脑缺血区及周围组织中可检测到BrdU阳性细胞,及少量BrdU/NSE、BrdU/GFAP双阳性细胞。结论移植的UCB-MSCs能逐渐向MCAO大鼠脑缺血区及周围迁移,并可分化为神经元细胞及神经胶质细胞,促进脑缺血性大鼠神经功能恢复。
Objective To investigate the therapeutic effect and mechanism of umbilical cord blood mesenchymal stem cells (UCB-MSCs) transplantation on neurological function recovery in rats with middle cerebral artery occlusion (MCAO). Methods Rats in MCAO model were randomly divided into experimental group (n = 20) and control group (n = 10). The rats in experimental group were injected UCB-MSCs labeled with BrdU (5-bromodeoxyuridine UU) In the control group, an equal volume of PBS was injected into the caudal vein to evaluate the neurological deficit in rats. The volume of infarct in rats was determined. BrdU positive cells, BrdU / NSE (neuron specific enolase), BrdU / GFAP (glial fibrillary acidic protein) double positive cells. Results Transplantation of UCB-MSCs could effectively reduce the neurological deficit scores of MCAO rats and decrease the volume of cerebral infarction in rats. BrdU positive cells and a small amount of BrdU / NSE were detected in cerebral ischemia and surrounding tissues of experimental rats, BrdU / GFAP double positive cells. CONCLUSION: Transplanted UCB-MSCs can gradually migrate into and around cerebral ischemia in MCAO rats, and can differentiate into neurons and glial cells, and promote the recovery of neurological function in cerebral ischemic rats.