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本世纪五十年代初,国外就有人开始用大鼠的游离肝细胞来研究肝细胞的生理功能。1954年Dulbeeo和Voget用胰蛋白酶消化肝脏,成功地得到了单个的肝细胞。Longmuir等又用此法研究了大鼠肝细胞的呼吸功能。此后,分离肝细胞的技术就得到了不断的改进和进一步的完善。1967年Howard首次报道了用胶元酶和透明质酸灌流并分离肝细胞的新方法,使游离肝细胞培养技术得到了广泛的应用,并发展成为一种简便、快速、经济的毒理学体外实验系统,为毒理学工作者所接受。
In the early 1950s, some people started to study the physiological function of hepatocytes by using rat’s free hepatocytes. In 1954 Dulbeeo and Voget digested the liver with trypsin and successfully obtained single hepatocytes. Longmuir et al used this method to study the respiratory function of rat hepatocytes. Since then, the technology of separating hepatocytes has been continuously improved and further improved. In 1967 Howard reported for the first time a new method of perfused and isolated hepatocytes with procollagen and hyaluronic acid, which made the technique of free hepatocyte culture widely used and developed into a simple, rapid and economical toxicological in vitro experiment System, accepted by toxicology practitioners.