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利用同轴电喷射技术,分别以含水甘油和溶解于N,N-二甲基乙酰胺(DMAC)的聚氨酯(PU)溶液为内、外相电喷液,制备中空PU微囊,考察了电喷液组成及流速对其结构的影响.结果表明,以80%(?)甘油溶液和10%(ω)PU溶液为内、外相电喷液,在流速分别为0.05和1 m L/h、电压约22 k V、针头距接收板48 cm、温度30℃、湿度约10%的条件下,可制备出结构均一的中空PU微囊.在上述电喷射条件下,将南极假丝酵母脂肪酶B(CALB)溶解于内相电喷液中,原位包埋在PU微囊腔室内.固定化CALB的活性回收率为游离酶的76.19%,重复使用10次后活性仍保持60%以上,60℃下的半衰期比游离酶提高了8倍.
Coaxial electrospray technique was used to prepare hollow PU microcapsules with aqueous glycerol and polyurethane (PU) dissolved in N, N-dimethylacetamide (DMAC) Liquid composition and flow rate on the structure of the system were investigated.The results showed that with 80% (?) Glycerol solution and 10% (ω) PU solution as internal and external phase EFGs, the flow rate was 0.05 and 1 m L / h, About 22 k V, the needle from the receiving plate 48 cm, the temperature 30 ℃, the humidity of about 10%, can be prepared uniform hollow PU microcapsules under the conditions of electrospray, Candida antarctica lipase B (CALB) was dissolved in the internal phase electrospray solution and embedded in PU microcapsule chamber in situ.The active recovery of immobilized CALB was 76.19% of that of free enzyme, and the activity remained above 60% after 10 repeated uses Half-life at 8 ° C is 8 times higher than that of free enzymes.