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目的探讨低剂量氢醌(HQ)对TK6淋巴母细胞的生物学性状及小分子非编码RNA(microRNA)-221(miR-221)表达的影响。方法磷酸盐缓冲液(PBS)溶解HQ,以PBS处理组为对照组,分别以2.5、5.0和10.0μmol/L HQ染毒TK6细胞为处理组。应用CCK-8试剂盒检测细胞活力和细胞增殖,通过磷脂结合蛋白(Annexin V)/碘化丙啶(PI)标记的流式细胞技术检测细胞凋亡,用实时荧光定量-聚合酶链反应(qRT-PCR)检测miR-221的表达改变。结果细胞增殖以48 h最为明显,2.5、5.0和10.0μmol/L组细胞增殖指数分别为1.33(P<0.05)、1.14(P<0.05)和1.12(P<0.05);miR-221表达改变以72 h最为明显,2.5、5.0、10.0μmol/L HQ组细胞miR-221表达量分别抑制了0.31倍(P<0.05)、0.39倍(P<0.05)和0.33倍(P<0.05)。结论低剂量HQ能抑制TK6细胞凋亡和miR-221的表达,促进细胞增殖。
Objective To investigate the effect of low dose hydroquinone (HQ) on the biological characteristics of TK6 lymphoblasts and the expression of small molecule non-coding RNA (miR-221). Methods HQ was dissolved in phosphate buffered saline (PBS) and treated with PBS as the control group. TK6 cells were treated with 2.5, 5.0 and 10.0 μmol / L HQ, respectively. Cell viability and cell proliferation were measured by CCK-8 kit. Apoptosis was detected by flow cytometry with Annexin V / Propidium iodide (PI) labeling. Real-time fluorescence quantitative polymerase chain reaction qRT-PCR) detection of miR-221 expression changes. The results showed that the cell proliferation was the most obvious at 48 h. The proliferation index of 2.5, 5.0 and 10.0 μmol / L groups were 1.33 (P <0.05), 1.14 (P <0.05) and 1.12 The expression of miR-221 was inhibited by 0.31-fold (P <0.05), 0.39-fold (P <0.05) and 0.33-fold (P <0.05), respectively, in cells treated with 2.5, 5.0 and 10.0 micromol / L HQ for 72 h. Conclusion Low-dose HQ can inhibit TK6 cell apoptosis and miR-221 expression and promote cell proliferation.