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介绍以氯敌鼠、华法令为代表的五种抗凝血杀鼠药的HPLC定性定量分析方法.在酸性条件下液-液提取和C_(18)柱固相萃取方法处理生物样品;选用C_(18)分离柱,流动相分别为:水、乙腈,邻酸二氢钾缓冲液(pH7.0)和甲醇/0.8%醋酸,均做梯度洗脱,DAD检测器,检测波长285nm,外标定量,测得血和肝中的提取回收率>60%.在0.01ug-1.0mg/ml范围内呈现良好的线性关系.适用于腐败生物样品抗凝血类杀鼠药中毒案件的检验及有关临床监测.
This paper introduces a method for the qualitative and quantitative analysis of five anticoagulant drugs, represented by chloropyril and warfarin, by liquid-liquid extraction and C 18 column solid-phase extraction (SPE) (18) column, the mobile phase was water, acetonitrile, potassium dihydrogen phosphate buffer (pH7.0) and methanol / 0.8% acetic acid were gradient elution, DAD detector, detection wavelength of 285nm, Quantitative determination of blood and liver extraction recovery rate of> 60% in the 0.01ug-1.0mg / ml range showed a good linear relationship .Applicable to biological samples of spoilage anti-clotting rodenticide poisoning test and related Clinical monitoring.