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目的:探讨细胞内铜/锌超氧化物岐化酶(copper zinc superoxide dismutase,Cu/Zn-SOD,SOD-1)在人胸主动脉夹层(humanthoracic aortic dissection,hTAD)中的表达情况及其在hTAD中的可能作用。方法:蛋白质印迹法(Western blot,WB)检测SOD-1在TAD和正常人胸主动脉(NA)中膜组织中的表达情况,免疫组织化学染色(immunohistochemistry,IHC)验证SOD-1在动脉壁中的表达和定位。结果:蛋白质印迹和免疫组化染色均显示SOD-1在TAD组表达量较NA组减低(P<0.05);免疫组化染色进一步显示,SOD-1主要位于主动脉壁中膜平滑肌细胞的胞质内,其在夹层主动脉壁中膜撕开处表达缺失。结论:SOD-1在TAD中表达量减少,可能由于参与氧化应激引起的脂质过氧化和炎症反应,以及细胞外基质(extracellular matrix,ECM)的降解等机制所致。
Objective: To investigate the expression of copper / zinc superoxide dismutase (Cu / Zn-SOD, SOD-1) in human thoracic aortic dissection (hTAD) Possible role in hTAD. METHODS: The expression of SOD-1 in TAD and normal human thoracic aortas (NA) was detected by Western blot (WB). The expression of SOD-1 in the arterial wall was detected by immunohistochemistry (IHC) In the expression and positioning. Results: Western blotting and immunohistochemistry showed that the expression of SOD-1 in TAD group was lower than that in NA group (P <0.05). Immunohistochemical staining further showed that SOD-1 mainly located in the aortic smooth muscle cells In vivo, it is missing in the dissection of the dissecting aortic wall. CONCLUSIONS: The decreased expression of SOD-1 in TAD may be due to its involvement in the mechanisms of oxidative stress-induced lipid peroxidation and inflammation, and the degradation of extracellular matrix (ECM).