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通过γ射线诱变,在水稻粳稻栽培品种9522中得到一个斑马叶突变体zebra leaf 1。为了研究zl1的功能,我们对突变体进行了形态学和细胞学的分析,同时也对此基因突变以后对叶绿体发育和光合作用的影响作了评价。突变体叶片上绿色和枯白色条纹相间,叶绿素含量显著的下降。电镜显示叶绿体类囊体的排列被打乱,变得杂乱无章。这表明zl1突变体在叶绿体发育过程中出现障碍。zl1基因的突变使得净光合速率显著的下降。参与光合作用的一些关键蛋白,比如核酮糖1,5-二磷酸羧化酶/加氧酶(Rubisco)、Rubisco活化酶、D1蛋白、CF1β亚基的表达量也显著的下调。但是,zl1突变体对外界环境非常敏感,有时会没有表型。
A zebra leaf mutant zebra leaf 1 was obtained in rice japonica cultivar 9522 by γ-ray mutagenesis. To investigate the function of zl1, we performed morphological and cytological analyzes of the mutants and also evaluated the effects of this gene mutation on chloroplast development and photosynthesis. Mutant leaves on the green and white stripes and white, chlorophyll content decreased significantly. Electron microscopy showed that the arrangement of chloroplast thylakoid was disrupted and became disorganized. This indicates that the zl1 mutant presents an obstacle in chloroplast development. Mutations in the zl1 gene led to a significant decrease in net photosynthetic rate. Some key proteins involved in photosynthesis, such as Rubisco, Rubisco activase, D1 protein and CF1β subunit, were also significantly down-regulated. However, zl1 mutants are very sensitive to the external environment, sometimes there is no phenotype.