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[目的]探讨脾酪氨酸激酶(spleen tyrosine kinase,SYK)在鼻咽癌中表达情况与鼻咽癌患者的预后的关系和调节机制。[方法]分别应用组织芯片技术、免疫组织/化学方法(immunohistochemistry,IHC)和甲基化特异性聚合酶链反应(methylation-specific PCR,MSP)检测SYK在鼻咽癌和鼻咽正常黏膜组织中的表达和启动子甲基化状态。[结果]在145例鼻咽癌组织中有57例高表达SYK,SYK在鼻咽癌组织中高表达与鼻咽癌患者的临床分期、T分期及复发呈正相关(P<0.05)。SYK高表达表达组和低表达组患者的5年生存率分别35.79%和63.50%,两组生存率差异有显著性(P<0.05)。SYK的表达和SYK启动子去甲基化成显著相关(R=0.711,P<0.001)。[结论]SYK在鼻咽癌组织中高表达并且与鼻咽癌患者的临床分期、T分期及复发明显正相关,其高表达提示预后不良。这些可能是SYK基因启动子去甲基化所致。
[Objective] To investigate the relationship between the expression of spleen tyrosine kinase (SYK) in nasopharyngeal carcinoma and the prognosis of patients with nasopharyngeal carcinoma and its regulatory mechanism. [Methods] Tissue microarray technique, immunohistochemistry (IHC) and methylation-specific PCR (MSP) were used to detect the expression of SYK in nasopharyngeal carcinoma and nasopharyngeal mucosa Expression and promoter methylation status. [Results] The high expression of SYK in 57 cases of nasopharyngeal carcinoma tissues and SYK in nasopharyngeal carcinoma tissues was positively correlated with clinical stage, T stage and recurrence (P <0.05). The 5-year survival rates of patients with SYK overexpression group and low expression group were 35.79% and 63.50% respectively, with significant difference between the two groups (P <0.05). SYK expression was significantly associated with SYK promoter demethylation (R = 0.711, P <0.001). [Conclusion] SYK is overexpressed in nasopharyngeal carcinoma and positively correlates with clinical stage, T stage and recurrence of nasopharyngeal carcinoma. The high expression of SYK suggests poor prognosis. These may be SYK gene promoter demethylation.