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目的观察植物黄酮3′,4′,5′,7′-四羟黄酮(luteolin)和3′-甲氧基,3,7,4′-三羟黄酮(geraldol)对白血病细胞HL-60氧化-还原电位的影响。方法四甲基偶氮噻唑蓝(MTT)法检测细胞活力变化;邻苯二醛(POT)比色法测定胞内还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)水平,计算GSH/GSSG及氧化-还原电位。结果10μmol/L的luteolin或Geraldol作用0h时细胞氧化-还原电位为-(295.5±31.2)mV,随后逐渐升高,分别作用4和2h后达到-(278.6±28.7)和-(279.6±26.3)mV,与对照组比较,差异有统计学意义(P<0.05);10μmol/LNAC干预能够降低luteolin和geraldol对HL-60细胞的增殖抑制效应,干预后其24h增殖抑制率分别由(25.4±1.8)%,(21.8±1.5)%降低至(16.8±1.1)%,(12.1±1.0)%,差异有统计学意义(P<0.05)。结论luteolin和ger-aldol能够明显升高HL-60细胞的氧化-还原电位,降低其GSH水平,可能在其抗肿瘤机制中发挥作用。
OBJECTIVE To observe the effects of 3, 4 ’, 5’, 7’-tetrahydroxyflavone and 3’-methoxy and 3,7,4’-geraldol on oxidation of HL-60 leukemia cells - Reduction potential effects. Methods The changes of cell viability were detected by MTT assay. The levels of intracellular glutathione (GSH) and glutathione (GSSG) were determined by POT colorimetric assay. , Calculated GSH / GSSG and oxidation-reduction potential. Results After the cells were treated with 10μmol / L luteolin or Geraldol for 0h, the oxidation-reduction potential of the cells was - (295.5 ± 31.2) mV and then increased gradually to (278.6 ± 28.7) and (279.6 ± 26.3) after 4 and 2 hours respectively. mV, compared with the control group, the difference was statistically significant (P <0.05); 10μmol / LNAC intervention could reduce the inhibitory effect of luteolin and geraldol on the proliferation of HL-60 cells, the inhibitory rate of 24h proliferation after intervention by (25.4 ± 1.8 ) And (21.8 ± 1.5)% to (16.8 ± 1.1)% and (12.1 ± 1.0)%, respectively, with statistical significance (P <0.05). Conclusion Luteolin and ger-aldol can significantly increase the oxidation-reduction potential and decrease the GSH level in HL-60 cells, which may play a role in its anti-tumor mechanism.