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目的:利用不同方法对白及基因组DNA进行提取,比较提取质量的差异,找到一种最适于白及基因组的DNA提取方法,以满足白及DNA条形码研究的需要。方法:采用十六烷基三甲基溴化铵(CTAB)法、十二烷基硫酸钠(SDS)法、碱裂解法和经本文改良的CTAB法提取白及叶片基因组DNA,利用琼脂糖凝胶电泳、紫外分光光度法及PCR扩增效果检测所提DNA的质量。结果:CTAB法、SDS法和改良CTAB法均能从白及中提取到基因组DNA,而碱裂解法几乎提取不到。其中改良CTAB法提取的DNA纯度和完整度均高于其他方法,PCR扩增效率达到100%,且扩增条带明亮清晰。结论:经本文改良的CTAB法是提取白及基因组DNA的最佳方法,该方法提取的基因组DNA适用于DNA条形码研究。
OBJECTIVE: To extract white and genomic DNA using different methods and to compare the differences in extraction quality to find a DNA extraction method that is most suitable for white and genome to meet the needs of research on white and DNA barcodes. Methods: CTAB method, sodium dodecyl sulfate (SDS) method, alkaline lysis method and modified CTAB method were used to extract white and leaf genomic DNA. Gel electrophoresis, UV spectrophotometry and PCR amplification to detect the quality of DNA. Results: Both CTAB method, SDS method and modified CTAB method could extract genomic DNA from white and medium, while alkaline lysis method could hardly extract it. Among them, the purity and integrity of DNA extracted by modified CTAB method were higher than those of other methods. The PCR amplification efficiency was 100%, and the amplified bands were bright and clear. Conclusion: The improved CTAB method is the best method to extract white and genomic DNA. The extracted genomic DNA is suitable for DNA barcoding.