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对15例HBsAg阳性(RPHA法)母亲的16例流产死胎开胸后心脏穿刺采血,并采集胎肝组织。应用ELISA检测胎儿血清HBsAg及IgM抗HBc,并检测胎儿血清HBV DNA多聚酶活力;免疫电镜法检测胎儿血清及肝匀浆中HBV抗原颗粒;将10％中性福尔马林固定、石蜡包埋的胎肝组织应用未标记的PAP法(DAKO PAP kit)检测肝细胞的HBsAg及HBcAg。结果以免疫电镜从其中7例胎儿血清及肝匀浆中查到HBsAg抗原颗粒,其中5例血
Twenty-six cases of stillbirth from 15 HBsAg positive (RPHA) mothers underwent heart puncture after thoracotomy and fetal liver tissue was collected. Fetal serum HBsAg and IgM anti-HBc were detected by ELISA and HBV DNA polymerase activity in fetal serum was detected. Immunocytochemistry was used to detect HBV antigen particles in fetal serum and liver homogenates. 10% neutral formalin fixed and paraffin-embedded HbAbs and HBcAg in hepatocytes were detected by unlabeled PAP method (DAKO PAP kit). Results Immuno-electron microscopy was used to detect HBsAg antigen particles from 7 cases of fetal serum and liver homogenate. Among them, 5 cases of blood