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应用淋巴细胞体外活化模型和流式细胞分析技术，观察异体角膜体外对人外周血Ｔ淋巴细胞ＣＤ２８分子表达的调节和ＦＫ５０６对Ｔ淋巴细胞ＣＤ２８表达的抑制作用．结果显示：空白对照组Ｔ淋巴细胞ＣＤ２８表达为２１．３％；受异体角膜或ＰＤＢ刺激后，Ｔ淋巴细胞ＣＤ２８表达分别为５２．４％和８１．４％，同时加入ＦＫ５０６的实验组，Ｔ淋巴细胞ＣＤ２８表达分别为２４．５％和４１．９％·结果表明异体角膜体外能够刺激Ｔ淋巴细胞ＣＤ２８分子表达，ＦＫ５０６体外能够明显抑制Ｔ淋巴细胞ＣＤ２８分子表达和活化．
In vitro activation of lymphocytes and flow cytometry were used to observe the regulation of CD28 expression on human peripheral blood T lymphocytes in vitro and the inhibitory effect of FK506 on CD28 expression on T lymphocytes. The results showed that CD28 expression of T lymphocytes was 21.3% in blank control group, CD28 expression of T lymphocytes was 52.4% and 81.4% respectively after being stimulated by allogeneic cornea or PDB, The expression of CD28 on lymphocytes was 24.5% and 41.9%, respectively. The results showed that allogeneic cornea could stimulate the expression of CD28 on T lymphocytes in vitro. FK506 could significantly inhibit the expression and activation of CD28 on T lymphocytes in vitro.