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为探讨吡喹酮杀灭斯氏狸殖吸虫的作用及其机制,我们于1985～1986年进行了体外实验观察,现将结果摘要报告如下。 从陕西宁强斯氏肺吸虫病流行区采集溪蟹,分离囊蚴。用腹腔注射法感染家犬,第25天和100天解剖。检出虫体经无菌Earle’s液(pH7.4)洗涤后移人RPMI1640培养液中常规培养6小时,再分别与含吡喹酮1μg/ml、10μg/ml和100μg/ml的培养基作用,并设正常对照。在体视显微镜下观察虫体活动,部分虫体
In order to explore the role and mechanism of praziquantel to kill the genus Trichogramma, we conducted an in vitro experiment from 1985 to 1986, and the summary of the results is reported below. Crabs were collected from the endemic area of Paragonimiasis in Ningqiang, Shaanxi, and the metacercariae were isolated. Infected dogs by intraperitoneal injection, 25 days and 100 days anatomy. After being washed by sterile Earle’s solution (pH7.4), the parasites were removed and cultured in RPMI1640 medium for 6 hours, then treated with 1μg / ml, 10μg / ml and 100μg / ml praziquantel respectively, And set up a normal control. Observation of body activity in the stereomicroscope, part of the body