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目的研究多壁碳纳米管(MWNTs)的光热效应及对人类肝癌HepG2细胞的杀灭作用。方法将多壁碳纳米管纯化、剪裁并用聚乙二醇(PEG)包覆,热电耦仪测定其光热效应。HepG2细胞分为对照组和3个剂量的MWNTs组,分别加入PBS和3个剂量(10,20,50μg·mL~(-1))MWNTs,与HepG2肝癌细胞共同培养24 h,在波长808 nm下,用红外光以强度2 W·cm~(-2)照射细胞5 min,用噻唑蓝比色法测定MWNTs对HepG2肝癌细胞的抑制作用;用AO-EB荧光染色测定MWNTs对HepG2肝癌细胞坏死的影响。结果在激光照射下,MWNTs迅速升温,温度可达56℃,与对照组相比,10μg·mL~(-1)MWNTs可明显抑制HepG2增殖(P<0.01),20μg·mL~(-1)MWNTs可杀灭HepG2细胞达80%以上,50μg·mL~(-1)MWNTs可杀灭HepG2细胞90%以上。结论多壁碳纳米管具有很强的光热转化效率,对HepG2肝癌细胞有强大的杀灭作用。
Objective To investigate the photothermal effects of multi-walled carbon nanotubes (MWNTs) and the killing effect on HepG2 cells. Methods Multiwalled carbon nanotubes were purified, cut and coated with polyethylene glycol (PEG), and their photothermal effects were measured with a thermocouple analyzer. HepG2 cells were divided into control group and three doses of MWNTs group, which were added into PBS and three MWNTs (10, 20, 50μg · mL -1) respectively, co-cultured with HepG2 hepatoma cells for 24 h, , The cells were irradiated with the intensity of 2 W · cm ~ (-2) by infrared light for 5 min, the inhibitory effect of MWNTs on HepG2 hepatoma cells was determined by thiazolyl blue colorimetry, and the apoptosis of HepG2 hepatoma cells was detected by AO-EB fluorescence staining Impact. Results Under the irradiation of laser, the MWNTs were rapidly heated up to 56 ℃. Compared with the control group, 10μg · mL -1 MWNTs significantly inhibited the proliferation of HepG2 (P <0.01), 20μg · mL -1 MWNTs can kill more than 80% HepG2 cells, and 50μg · mL -1 MWNTs can kill more than 90% of HepG2 cells. Conclusion Multi-walled carbon nanotubes have a strong photothermal conversion efficiency and have a powerful killing effect on HepG2 hepatoma cells.