论文部分内容阅读
目的研究合体滋养层细胞微粒(syncytiotrophoblast microparticles STBM)的体外制备方法,并检测其纯度。方法收集两只足月正常健康剖宫产孕妇胎盘,将胎盘组织用机械方法打碎,运用差速离心法体外制备STBM;以胎盘碱性磷酸酶(placental alkaline-phosphatase PALP)作为膜标志酶,采用酶联免疫分析法检测STBM制剂的PALP的含量,确定STBM制剂的纯度。结果正常健康剖宫产孕妇胎盘中PLAP含量为56μg/ml,由其制备而来的STBM中PALP含量为900μg/ml,STBM较相应的胎盘组织纯化了16.07倍。结论取材于足月正常健康剖宫产孕妇胎盘组织,运用机械打碎方法及差速离心法,可以制备出高纯度的STBM,该方法简便易行,可作为一种高效、经济的体外制备STBM的方法。
Objective To study the in vitro preparation of syncytiotrophoblast microparticles STBM and its purity. Methods Placenta of placental alkaline phosphatase (placental alkaline phosphatase PALP) was prepared from placental tissue from two full-term cesarean section pregnant women with placental alkaline phosphatase (PALP) by mechanical centrifugation. The content of PALP in STBM preparation was detected by enzyme-linked immunosorbent assay and the purity of STBM preparation was determined. Results The placental content of placenta in normal healthy cesarean section pregnant women was 56μg / ml. The content of PALP in STBM was 900μg / ml and the STBM was 16.07 times more than the corresponding placenta. Conclusion The placenta tissue of pregnant women with full-term normal cesarean section can be prepared by mechanical disintegration method and differential centrifugation method, which can be used to prepare high-purity STBM. The method is simple and easy to use and can be used as an efficient and economical method for the preparation of STBM Methods.