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对虹鳟(Salmo gairdneri)排卵前后血清17β-雌二醇(17β-E_2),睾酮(T)和17α-羟-20β-双氢孕酮(17α20βP)的含量进行了测定。17β-E_2从排卵前15天的18.1ng/ml急骤下降到排卵前3—6天的2ng/ml,并继续下降,至排卵时仅为0.9ng/ml。睾酮在虹鳟雌鱼血清中的含量很高,血清浓度从排卵前9天的峰值水平143.3ng/ml缓慢下降,排卵时为24.3ng/ml。17α20βP的血清浓度变化明显,排卵前15天,该激素浓度接近于基线水平或甚至低得不能被检测,排卵前9天开始迅速上升,到排卵前3天达到峰值350.6ng/ml,排卵时为302.2ng/ml。本研究进一步证实了17α20βP在鲑鳟鱼类卵母细胞最后成熟过程中的生理作用,是一种诱发卵母细胞成熟的类固醇激素。临排卵前,血清17β-E_2浓度的下降,可能调节了17α20βP大量分泌的时间;17α20βP的大量分泌是卵母细胞达到最后成熟和排卵不可缺少的一环。注射合成的大麻哈鱼(Oncorhynchus keta)促性腺激素释放激素(s-GnRH)及其类似物(s-GnRH-A),诱发虹鳟血清类固醇激素17β-E_2,睾酮和17α20βP的变化趋势与自然排卵时的变化相类似。经注射药物诱发排卵的鱼,较对照组提早一周排卵,而且排卵较同步和集中。
The contents of 17β-estradiol (17β-E2), testosterone (T) and 17α-hydroxy-20β-dihydroprogesterone (17α20βP) in rainbow trout (Salmo gairdneri) before and after ovulation were measured. 17β-E 2 abruptly dropped from 18.1 ng / ml 15 days prior to ovulation to 2 ng / ml 3-6 days prior to ovulation and continued to decrease to only 0.9 ng / ml at ovulation. Testosterone was found to be highly expressed in serums of rainbow trout females with a serum concentration of 14.3 ng / ml when ovulating, from a peak of 143.3 ng / ml at 9 days prior to ovulation. Serum 17α20βP concentration changes significantly, 15 days before ovulation, the hormone concentration close to the baseline level or even low can not be detected, 9 days before ovulation began to rise rapidly to 3 days before ovulation peak 350.6ng / ml ovulation 302.2 ng / ml. This study further confirms the physiological role of 17α20βP in the eventual maturation of salmon-type oocytes, a steroid hormone that induces oocyte maturation. Pro-ovulation, the decline in serum 17β-E 2 levels may regulate the 17α20βP large secretion of time; 17α20βP large secretion of oocytes to achieve the final maturation and ovulation an indispensable link. The tendency of the serum steroid hormones 17β-E_2, testosterone and 17α20βP induced by Oncorhynchus keta injection of s-GnRH and its analog (s-GnRH-A) When the change is similar. Ovulation induced by injection of fish, compared with the control group ovulation a week earlier, and ovulation more synchronized and concentrated.