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1906年伟大的俄国植物学家М.С.Цвет氏在“德国植物学会通报”(Berichte der Deutschen bo-tanischen Gesellschaft)杂志上发表了一篇论文,这篇论文虽然只有8页左右,但已经说明了色層分离法的基本原理。今天我们广泛的应用色層分离法的同时,应该紀念这位伟大的俄国植物学家М.С.Цвет氏——色層分离法的奠基人。为了便于说明起见,这里简述一下М.С.Цвет氏在这方面的工作。М.С.Цвет氏曾用酒精或醚浸漬干的綠色树叶,浸出了所有的色素成份,使抽出液呈现似叶綠素的暗綠色,叶中的胡萝卜烃类色素的黄色隐而不显。若在同样情况下以石油醚为溶剂,则抽出液显黄色,以胡萝卜烃类色素为主成份,但不含叶綠素。若于石油醚中加入少量之酒精,即可抽出所有的叶綠素。对于这种现象,必须提出一个问题“为什么酒精可以增进叶綠素的溶解度?”
In 1906 the great Russian botanist М.С.Цвет published a paper in the journal “Brichte der Deutschen bo-tanischen Gesellschaft”. Although this paper was only about eight pages long, The basic principle of chromatographic separation has been described. Today, when we apply the chromatographic separation method extensively, we should commemorate the great Russian botanist М.С.Цвет - the founder of chromatographic method. For the sake of illustration, the work of М.С.Цвет in this respect is briefly described here. М.С.Цвет has impregnated dried green leaves with alcohol or ether, leaching all the pigment components, making the extract appear dark green with chlorophyll, and the yellow color of carrot hydrocarbon pigments in leaves is not obvious. If petroleum ether is used as the solvent under the same conditions, the extracted liquid is yellow, and carrot hydrocarbon pigment is the main ingredient, but it does not contain chlorophyll. If you add a small amount of alcohol to petroleum ether, you can extract all the chlorophyll. For this phenomenon, a question must be asked “Why alcohol can increase the solubility of chlorophyll?”