论文部分内容阅读
应用原位杂交技术检测了２８ 例原发性肝细胞癌（ＨＣＣ）中Ｐ１６／ＣＤＫＮ２ 基因ｍ ＲＮＡ的表达， 并用免疫组织化学法检测了Ｐ１６ 蛋白及细胞周期蛋白依赖性激酶４ （ｃｙｃｌｉｎ－ｄｅｐｅｎｄｅｎｔｋｉｎａｓｅ ４， ＣＤＫ４） 蛋白的表达。结果显示： ７例ＨＣＣ的Ｐ１６基因ｍ ＲＮＡ为阴性， １３ 例为异质性减弱， ８ 例为保留表达。ｍ ＲＮＡ阳性的ＨＣＣ中， 均可见到Ｐ１６ 蛋白表达。Ｐ１６／ＣＤＫＮ２ 基因表达与ＨＣＣ的分级、分化及转移无关。２８ 例ＨＣＣ中共有６ 例出现ＣＤＫ４ 蛋白阳性，这６ 例ＨＣＣ均有不同程度的Ｐ１６ 蛋白表达， 提示ＣＤＫ４ 蛋白表达可能是灭活Ｐ１６ 蛋白的又一途径。
The expression of P16/CDKN2 m RNA in 28 cases of primary hepatocellular carcinoma (HCC) was detected by in situ hybridization, and P16 protein and cyclin-dependent kinase 4 (cyclin-dependent kinase 4) were detected by immunohistochemistry. , CDK4) Protein Expression. The results showed that: 7 cases of HCC P16 gene m RNA negative, 13 cases of heterogeneity weakened, 8 cases were reserved expression. In the m RNA-positive HCC, P16 protein expression was observed. P16/CDKN2 gene expression was not associated with HCC grading, differentiation, and metastasis. A total of 6 cases of 28 cases of HCC were found to have CDK4 protein positive. These 6 cases of HCC all have different levels of P16 protein expression, suggesting that CDK4 protein expression may be another way to inactivate P16 protein.