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将人肝细胞生长因子（ＨＧＦ）ｃＤＮＡ（２．２ｋｂ）经ＤＮＡ连接酶插入到ｐＥＥ１４质粒的多克隆位点上，构建了ｐＥＥ１４／人ＨＧＦ（１１．４５ｋｂ）真核细胞表达质粒。用Ｌｉｐｏｆｅｃｔｉｎ将ｐＥＥ１４／ＨＧＦ质粒转导至ＣＨＯ细胞表达，表达ｐＥＥ１４／人ＨＧＦ的ＣＨＯ细胞的培养上清液能促进原代培养大鼠细胞的ＤＮＡ合成，ＣＨＯ细胞表达和合成的人ＨＧＦ的水平高于２９３和ＮＳＯ细胞。
The human hepatocyte growth factor (HGF) cDNA (2.2 kb) was inserted into the multiple cloning site of the pEE14 plasmid by DNA ligase to construct a eukaryotic expression plasmid pEE14 / human HGF (11.45 kb). Transduction of pEE14 / HGF plasmids into CHO cells with Lipofectin The culture supernatant of CHO cells expressing pEE14 / human HGF promoted DNA synthesis in primary cultured rat cells, and the level of human HGF expressed and synthesized in CHO cells was high 293 and NSO cells.