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目的：为了了解血栓形成后血管内皮细胞释放内皮依赖性舒张因子（ＥＤＲＦ）的功能是否改变，实验观察了血栓形成对乙酰胆碱（Ａｃｈ）内皮依赖性舒张血管作用的影响．方法：大鼠足底皮下局部注射０．５％角叉菜胶（ｃａｒｒａｇｅｅｎａｎ），制备大鼠尾动脉血栓形成模型．将血栓形成部位的血管制成离体血管条，置于充满３７℃Ｋｒｅｂｓ液的浴槽中，以１Ｏ￣（－７）ｍｏｌ／Ｌ去甲肾上腺素（ＮＥ）引起血管条收缩，观察Ａｃｈ和硝酸甘油的作用。结果：血栓形成开始后６～８ｈ，Ａｃｈ（１０￣（－８），１０￣（－７），１０￣（－６）ｍｏｌ／Ｌ）剂量依赖性地舒张尾动脉血管条（２４．８±３．３％，４２．１±４．７％，６６．６±３．８％，Ｐ＜０．０５）；血栓形成开始后８～１６ｈ，Ａｃｈ剂量依赖性地舒张血管条作用明显减弱（９．３±１．５％，１５．９±２．９％，１９．７±３．４％，Ｐ＜０．００１）；血栓形成开始１６ｈ后，血管条对１０￣（－７）ｍｏｌ／ＬＮＥ的反应性消失；机械法擦去尾动脉内皮后，Ａｃｈ剂量依赖性地舒张血管条的作用亦明显减弱（Ｐ＜０．００１），与血栓形成后８～１６ｈ时的Ａｃｈ作用之间无显著差别（Ｐ＞０．０５）．去内皮或血栓形成后（６～８ｈ，８～?
OBJECTIVE: To investigate whether the function of endothelium-dependent relaxing factor (EDRF) in vascular endothelial cells after thrombosis is changed, the effect of thrombosis on the endothelium-dependent relaxation of blood vessels by acetylcholine (Ach) was observed. METHODS: Rats were subcutaneously injected with 0.5% carrageenan subcutaneously to prepare rat tail artery thrombosis model. The blood vessels of the thrombosis site were made into ex vivo vascular strips and placed in a bath filled with Krebs solution at 37 ° C to induce contractions of the vascular strips with 10 ~ (-7) mol / L norepinephrine (NE). Ach and nitric acid The role of glycerol. Results: Ach (10 ~ (-8), 10 ~ (-7), 10 ~ (-6) mol / L) dose-dependently dilated the tail artery strips at 24 h 3.3%, 42.1 ± 4.7%, 66.6 ± 3.8%, P <0.05). The dose-dependent relaxation of Ach was significantly attenuated by Ach 8 to 16 hours after the start of thrombosis 9.3 ± 1.5%, 15.9 ± 2.9%, 19.7 ± 3.4%, P <0.001). After 16 h of thrombosis, / LNE reactivity disappeared; Ach dose-dependent relaxation of the vascular strips was also significantly attenuated (P <0.001) after mechanical wiping off of the tail artery endothelium, and between Ach effect at 8-16 h after thrombosis No significant difference (P> 0.05). After the endothelium or thrombosis (6 ~ 8h, 8 ~?