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目的:离体条件下葡萄糖孵育SD大鼠乳鼠心脏成纤维细胞,观察罗格列酮干预后,对乳鼠心脏成纤维细胞表达Ⅰ型前胶原的影响,从而探讨罗格列酮在糖尿病心肌病心肌纤维化中的作用机制。方法:提取、培养新生SD大鼠心脏成纤维细胞,设对照组(葡萄糖5.5mmol.L-1);低糖5.5mmol.L-1+罗格列酮组;高糖30mmol.L-1组;高糖30mmol.L-1+罗格列酮组。应用Real time PCR法测定心脏成纤维细胞Ⅰ型前胶原-mRNA表达;Western blot检测Ⅰ型前胶原蛋白质的表达。结果:罗格列酮对高糖孵育下心脏成纤维细胞Ⅰ型前胶原-mRNA/protein表达的影响:与对照组相比,高糖刺激下Ⅰ型前胶原-mRNA/protein表达均增加(P<0.01)。与高糖30mmol.L-1组相比,高糖30mmol.L-1+罗格列酮组Ⅰ型前胶原-mRNA/protein表达降低(P<0.05)。结论:罗格列酮抑制了高血糖环境内心脏成纤维细胞胶原的合成,可能在抑制心肌纤维化的过程中发挥了作用。
OBJECTIVE: To investigate the effect of rosiglitazone on the expression of type Ⅰ procollagen in neonatal rat cardiac fibroblasts after glucose was incubated with glucose in vitro. The effects of rosiglitazone on cardiac fibroblasts in diabetic rat myocardium Mechanism of myocardial fibrosis. Methods: Newborn SD rat cardiac fibroblasts were isolated and cultured. The control group (glucose 5.5 mmol.L-1), low glucose 5.5 mmol.L-1 + rosiglitazone group, high glucose 30 mmol.L-1 group High glucose 30mmol.L-1 + rosiglitazone group. Real-time PCR method was used to detect the expression of type Ⅰ procollagen-mRNA in cardiac fibroblasts. The expression of type Ⅰ procollagen was detected by Western blot. RESULTS: The effect of rosiglitazone on type Ⅰ procollagen-mRNA / protein expression in cardiac fibroblasts induced by high glucose: Compared with the control group, the expression of type Ⅰ procollagen-mRNA / protein increased (P <0.01). Compared with the high glucose 30 mmol.L-1 group, the type Ⅰ procollagen-mRNA / protein expression in high glucose 30 mmol.L-1 + rosiglitazone group decreased (P <0.05). CONCLUSION: Rosiglitazone inhibits collagen synthesis in cardiac fibroblasts in a hyperglycemic environment and may play a role in the inhibition of myocardial fibrosis.