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目的 研究严重急性呼吸综合征 (SARS)患者淋巴细胞及其亚群的表型变化 ,以期为SARS的诊断治疗提供新的监测指标。方法 采用多色流式细胞术结合血液分析仪测定 38例SARS患者外周血淋巴细胞及其亚群的表达情况。结果 84%的患者淋巴细胞绝对计数降低。各类淋巴细胞亚群 [T/B淋巴细胞、自然杀伤 (NK)细胞 ]的绝对计数均有不同程度降低 ,其中T细胞降低者最多 (占 95 %) ;在T细胞中 ,T辅助 /诱导细胞 (T4 )降低者 (10 0 %)多于T抑制 /NK细胞 (T8)降低者 (87%)。从各类淋巴细胞分布看 ,T淋巴细胞百分率下降者最多 (5 8%) ;B细胞和NK细胞百分率以相对正常或升高为主 ,相对下降者均很少 ,分别占 2 6 %和 5 3%;在T细胞中 ,T4 细胞的百分率降低者 (82 %)高于T8细胞降低者 (34 %) ,两者比值降低者占44 %。结论 SARS患者的淋巴细胞总数及其各亚群均受到破坏 ,以T淋巴细胞受损为主 ,且T4 细胞较T8细胞受损严重 ;淋巴细胞亚群的绝对计数变化较相对计数更明显。
Objective To study the phenotypic changes of lymphocytes and their subpopulations in patients with severe acute respiratory syndrome (SARS) and to provide new monitoring indicators for the diagnosis and treatment of SARS. Methods The expression of peripheral blood lymphocytes and their subpopulations in 38 patients with SARS were determined by multicolor flow cytometry and hematology analyzer. Results 84% of patients with decreased absolute lymphocyte count. The absolute counts of all kinds of lymphocyte subsets [T / B lymphocytes and natural killer (NK) cells] decreased to different extents, among which the decrease of T cells was the most (95%); in T cells, T helper / Decreased cells (T4) (10 0%) were more than T suppressor / NK cells (T8) decreased (87%). According to the distribution of various lymphocytes, the percentage of T lymphocytes was the most (58%). The percentage of B cells and NK cells was relatively normal or increased, while the relative decrease was only 26% and 5% respectively 3%. In T cells, the percentage of T4 cells (82%) was higher than that of T8 cells (34%), the ratio of them was 44%. Conclusions The total number of lymphocytes and their subgroups in SARS patients were destroyed. The main damage was T lymphocytes, and the damage of T4 cells was more serious than that of T8 cells. The absolute count change of lymphocyte subsets was more obvious than the relative counts.