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目的:为了探讨临床验方和合疏养方治疗四氯化碳(CCl4)致肝损伤大鼠模型肝纤维化疗效的量效关系。方法:采用正交试验,SD大鼠60只,雌雄各半,分为6个组,空白对照组、模型组、阳性药物组、和合疏养方(HHSYF)低剂量组、HHSYF中剂量组及HHSYF高剂量组。空白对照组采用慢性束缚应激+过度运动+饮食失节的方法造模4周后,予蒸馏水灌胃至8周。模型组及阳性药物组、HHSYF组采用慢性束缚应激+过度运动+饮食失节的方法同时予腹腔注射30%CCl4花生油溶液造模4周后,模型组予蒸馏水灌胃至8周;阳性药物组予联苯双酯(50 mg/kg)灌胃治疗至8周;HHSYF低剂量组、中剂量组及高剂量组分别按16.6、41.5、83.0 g/kg剂量灌胃治疗至8周。结果:随着HHSYF组的剂量增长,HHSYF在恢复肝功能、促进肝纤维化逆转的疗效递增,HHSTF中剂量组与高剂量组在肝功能恢复、肝纤维化恢复等方面优于空白对照组、模型组与阳性药物对照组。结论:和合疏养方对于改善肝功能、逆转肝纤维化具有较好的功效,并呈剂量-疗效递增关系。
OBJECTIVE: To investigate the dose-response relationship between clinical prescription and Hegan-Prescription for the treatment of hepatic fibrosis induced by carbon tetrachloride (CCl4) in rats with liver injury. METHODS: Sixty SD rats were divided into 6 groups according to orthogonal test: control group, model group, positive drug group, low dose HHSYF group, middle dose HHSYF group, HHSYF high-dose group. The blank control group was treated with chronic restraint stress + over-exercise + dietary disorders for 4 weeks, then distilled water was given to 8 weeks. The model group and the positive drug group, HHSYF group were treated with 30% CCl4 peanut oil solution by intraperitoneal injection of chronic restraint stress + excessive exercise + diet for 4 weeks. The model group was given distilled water for 8 weeks. The positive drug group Bifendate (50 mg / kg) was given intragastrically for 8 weeks. The HHSYF low dose group, middle dose group and high dose group were treated with 16.6, 41.5 and 83.0 g / kg respectively by intragastric administration for 8 weeks. Results: With the increase of dose in HHSYF group, the effect of HHSYF in restoring liver function and promoting the reversal of hepatic fibrosis increased. Compared with the blank control group, HHSSTF middle dose group and high dose group had better liver function recovery and liver fibrosis recovery, Model group and positive drug control group. Conclusion: Heshuxugan Recipe has good effect on improving liver function and reversing hepatic fibrosis, and has a dose-response relationship.