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The acoustic radiation force on a fluid sphere immersed in water between two boundaries given by a Gaussian beam is theoretically and numerically investigated in this work.Based on the finite series method,the Gaussian beam is expressed in terms of Bessel function and a weighting parameter.The effects of the two boundaries conceed in our study is worked out by the image theory.This work also provides a reference when considering the effects of certain factors such as the radius of the sphere and the distance between the sphere and two boundaries.The contrast with the acoustic radiation force on a fluid sphere near only one boundary is also made in this paper.Our study can offer a theoretical basis for acoustics manipulation,acoustic sensors in the field of biomedical ultrasound and material science.