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目的:研究食管癌相关基因2(ECRG2)短串联重复序列(STR)多态性与新疆哈萨克族和汉族食管癌易感性的关系。方法:新疆地区食管癌哈萨克族94例,汉族84例;人群对照哈萨克族100例,汉族53例,采用聚合酶链反应单链构象多态性分析(PCR-SSCP)技术检测研究对象的ECRG2 STR基因型。结果:ECRG2 STR呈多态性,可分为3种类型:TCA3/TCA3,TCA4/TCA4和TCA3/TCA4.3种基因型在有转移的食管癌中分布为58.8%,7.8%和33.3%,在无转移的食管癌中分布为14.2%,40.9%和44.9%,两者相比有显著差异(x~2=40.74,v=2,P<0.01,),携带TCA3/TCA3基因型个体更容易发生转移。汉族人群中,携带TCA3/TCA3基因型个体患食管癌的风险比TCA4/TCA4基因型个体高3.25倍(95%的可信区间为1.25-8.45);在哈萨克族人群中,携带TCA3/TCA3基因型个体患食管癌的风险比TCA4/TCA4基因型个体高4.06倍(95%的可信区间为1.69-9.74).结论:携带ECRG2基因TCA3/TCA3基因型个体与TCA4/TCA4基因型个体比较,增加了患食管癌和发生转移的风险。
OBJECTIVE: To study the relationship between short-term tandem repeat (STR) polymorphisms of esophageal cancer-associated gene 2 (ECRG2) and susceptibility to esophageal cancer in Xinjiang Kazak and Han populations. Methods: There were 94 Kazak patients with esophageal cancer and 84 Han patients in Xinjiang; population control Kazakh 100 and Han 53; PCR-SSCP technique was used to detect ECRG2 STR genotype. RESULTS: ECRG2 STR was polymorphic and could be divided into 3 types: TCA3/TCA3, TCA4/TCA4 and TCA3/TCA 4.3 genotypes were distributed in 58.8%, 7.8% and 33.3% of metastatic esophageal cancers. In the absence of metastatic esophageal cancer, the distributions were 14.2%, 40.9%, and 44.9%, which were significantly different (x~2=40.74, v=2, P<0.01). Individuals carrying the TCA3/TCA3 genotype were more It is easy to transfer. In the Han nationality, individuals carrying the TCA3/TCA3 genotype have a 3.25-fold higher risk of esophageal cancer than individuals of the TCA4/TCA4 genotype (95% confidence interval 1.25-8.45); in the Kazakh population, carry the TCA3/TCA3 gene Individuals with esophageal cancer were 4.06 times more likely to develop esophageal cancer than individuals with the TCA4/TCA4 genotype (95% confidence interval 1.69 to 9.74). Conclusions: Individuals carrying the ECRG2 gene TCA3/TCA3 genotype were compared with individuals with the TCA4/TCA4 genotype. Increased risk of esophageal cancer and metastasis.