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目的 :探讨奥美拉唑对大鼠胃粘膜的保护作用。方法 :在乙醇诱导大鼠胃粘膜损伤前 ,预先给予奥美拉唑、L 硝基 精氨酸甲酯 (L NAME)静脉注射。测定胃粘膜血流量 (GMBF)、胃液 pH和胃粘膜NO-2 /NO-3 含量 ,并观察胃粘膜损伤指数 (Ulcerindex ,UI)、溃疡坏死组织和中性粒细胞浸润严重程度的变化。结果 :与模型损伤组比 ,奥美拉唑组大鼠UI明显降低 (P <0 .0 1) ,溃疡坏死组织和中性粒细胞浸润程度明显减轻 (P <0 .0 1)。预先用L NAME处理后 ,奥美拉唑保护胃粘膜损伤的作用明显减弱。静脉注射奥美拉唑 ,可增加GMBF和胃粘NO-2 /NO-3 含量 ,L NAME可逆转这种作用 ,但对奥美拉唑抑制酸分泌作用无明显影响。结论 :奥美拉唑对大鼠胃粘膜具有重要的保护作用 ,一氧化氮介导了这种作用
Objective: To investigate the protective effect of omeprazole on gastric mucosa in rats. Methods: Omeprazole and L-NAME were given intravenously before alcohol-induced gastric mucosal injury in rats. Gastric mucosal blood flow (GMBF), gastric pH and gastric mucosal NO-2 / NO-3 content were measured. The gastric mucosal injury index (Ulcerindex, UI), ulcer necrosis and neutrophil infiltration severity were also observed. Results: Compared with the model group, the UI of omeprazole group was significantly decreased (P <0.01), and the degree of infiltration of necrotic tissue and neutrophil in ulcer group was significantly reduced (P <0.01). The effect of omeprazole in protecting gastric mucosal lesions was significantly diminished after pretreatment with L NAME. Intravenous omeprazole increased GMBF and gastric mucosal NO-2 / NO-3 content, L NAME can reverse this effect, but no significant effect on the inhibition of acid secretion of omeprazole. Conclusion: Omeprazole has an important protective effect on gastric mucosa in rats, and nitric oxide mediates this effect