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目的 研究瘤内单纯注射32 P胶体和先注射聚合白蛋白 (MAA) ,再注射32 P胶体两种不同给药方法的32 P在瘤外各组织器官的动态分布 ,探讨不同剂量MAA的阻滞效果及MAA颗粒数量和32 P胶体应用剂量的相关关系。方法 在Balb/c小鼠右侧胸前皮下接种H2 2 肝癌细胞 ,10天后接种部位长出直径约 1cm的肿瘤。随机将其分为 4组 :第 1组只注射32 P胶体 1 85MBq ;第 2组先注射 1× 10 4颗粒MAA ,再注射32 P胶体 1 85MBq ;第 3组先注射 1× 10 5颗粒MAA ,再注射32 P胶体 1 85MBq ;第 4组先注射 1× 10 5颗粒MAA ,再注射32 P胶体 18 5MBq。注射后 2 4小时、第 8天和第 16天时处死小鼠 ,测定心、肝、肾、肺和骨骼的放射性。结果 瘤内注射32 P胶体时 ,32 P可向全身其他器官扩散 ;当向肿瘤内注射的32 P胶体剂量相同时 ,预先注入 1× 10 4颗粒MAA和 1× 10 5颗粒的两组小鼠 ,其体内32 P的分布均比未注射MAA的一组小鼠要少 ,其中 1× 10 5颗粒MAA组的小鼠 ,32 P体内分布又比 1× 10 4颗粒MAA组少 ;当预先注入的MAA颗粒数量相同时 ,注射的32 P胶体剂量增加 ,体内分布亦随之增加。结论 与单纯瘤内注射32 P胶体相比 ,先在瘤内注入MAA ,再注入32 P胶体 ,MAA可以有效阻止32 P胶体的全身扩散 ,使32 P胶体能够较长时间的滞留在肿瘤
Objective To investigate the dynamic distribution of 32 P in tumor tissues and tumor tissues by intratumoral injection of 32 P colloid and injection of poly(alarbumin) (MAA) before injection of 32 P colloid, and to explore the block of different doses of MAA. Effect and correlation between MAA particle number and 32 P colloid application dose. Methods H2 2 liver cancer cells were inoculated subcutaneously on the right chest of Balb/c mice. After 10 days, tumors with a diameter of about 1 cm were grown on the site of inoculation. They were randomly divided into 4 groups: Group 1 injected only 32 P colloids 1 85 MBq; Group 2 injected 1×10 4 particles of MAA first, and then injected 32 P colloids 1 85 MBq; Group 3 first injected 1×10 5 particles of MAA. The injection of 32 P colloidal 185MBq; group 4 first injection of 1 × 10 5 particles of MAA, and then injection of 32 P colloidal 18 5MBq. Mice were sacrificed 24 hours, 8 days, and 16 days after injection and the radioactivity of heart, liver, kidney, lung, and bone was measured. Results When intratumoral injection of 32 P colloids, 32 P could spread to other organs of the body; when the dose of 32 P colloids injected into the tumor was the same, 1×10 4 particles of MAA and 1×10 5 particles of two groups of mice were injected in advance. The distribution of 32 P in vivo was lower than that of mice in which MAA was not injected. In mice of 1×10 5 MAA group, the distribution of 32 P was less than that of 1×10 4 MAA group; When the number of MAA particles is the same, the injected dose of 32 P colloids increases, and the distribution in vivo also increases. Conclusion Compared with intratumoral injection of 32 P colloid, MAA was first injected into the tumor and then 32 P colloid was injected. MAA can effectively prevent the systemic diffusion of colloidal 32 P, and 32 P colloids can stay in the tumor for a longer period of time.