,High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and flu

• 作 者: Wei Zhang Yanxiu Li Van Phuc Nguyen Ziyi Huang Zhipeng Liu Xueding Wang Yannis M.Paulus
• 机 构: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48105, USA“,”Institution
• 出 处: 光：科学与应用（英文版）
• 发表日期: 2018年6期

Photoacoustic microscopy (PAM) is an emerging imaging technology that can non-invasively visualize ocular structures in animal eyes.This report describes an integrated multimodality imaging system that combines PAM,optical coherence tomography (OCT),and fluorescence microscopy (FM) to evaluate angiogenesis in larger animal eyes.High-resolution in vivo imaging was performed in live rabbit eyes with vascular endothelial growth factor (VEGF)-induced retinal neovascularization (RNV).The results demonstrate that our multimodality imaging system can non-invasively visualize RNV in both albino and pigmented rabbits to determine retinal pathology using PAM and OCT and verify the leakage of neovascularization using FM and fluorescein dye.This work presents high-resolution visualization of angiogenesis in rabbits using a multimodality PAM,OCT,and FM system and may represent a major step toward the clinical translation of the technology.