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AIM:To evaluate the effects of angiopoietin-1(Ang-1) on adhesion of gastric cancer cell line BGC-823 and expression of integrinβ1,CD44V6,urokinase-type plasminogen activator(uPA)and matrix metalloproteinase-2(MMP-2). METHODS:BGC-823 cells were transfected transiently with adenovirus-Ang-1(Ad-Ang-1).Cells transfected transiently with adenovirus-green fluorescent protein (Ad-GFP)and untransfected cells were used as a negative and blank control group,respectively.The cell adhesion rate between cell and extracellular matrix(ECM)was determined by cell adhesion assay. To investigate whether Ang-1 could reinforce gastric carcinoma metastasis,we performed migration and invasion assays in BGC-823 cells.The mRNA and protein expression of integrinβ1,CD44V6,uPA and MMP-2 were detected by reverse transcription polymerasechain reaction and Western blotting,respectively.The expression of integrinβ1 and CD44V6 was measured by immunohistochemistry. RESULTS:BGC-823 cells were transfected successfully. The adhesion rate increased significantly in the Ad- Ang-1 group(P<0.05).The Ad-Ang-1-transfected group had a significant increase in migration and invasion compared with that of the mock-transfected and Ad-GFP groups.The mRNA and protein expression of integrinβ1,CD44V6,uPA and MMP-2 in the Ad-Ang-1 group was higher than that in the Ad-GFP and blank control groups(P<0.05).Compared with mock- transfected and Ad-GFP groups,integrinβ1 and CD44V6 expression intensity greatly increased(P<0.05). CONCLUSION:Transfection of Ang-1 into human gastric cancer cell line BGC-823 can significantly increase expression of integrinβ1 and CD44V6,by which cell adhesion and metastasis to the ECM are promoted.
AIM: To evaluate the effects of angiopoietin-1 (Ang-1) on adhesion of gastric cancer cell line BGC-823 and expression of integrin β1, CD44V6, urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-2 METHODS: BGC-823 cells were transfected transiently with adenovirus-Ang-1 (Ce-transfected transiently with adenovirus-green fluorescent protein (Ad-GFP) and untransfected cells were used as a negative and blank control group , respectively.The cell adhesion rate between cell and extracellular matrix (ECM) was determined by cell adhesion assay. To investigate whether Ang-1 could reinforce gastric carcinoma metastasis, we performed migration and invasion assays in BGC-823 cells. The mRNA and protein Expression of integrinβ1, CD44V6, uPA and MMP-2 were detected by reverse transcription polymerase chain reaction and Western blotting, respectively. The expression of integrinβ1 and CD44V6 was measured by immunohistochemistry. RESULTS: BGC-823 cells were transfected successfully . The adhesion rate increased significantly in the Ad-Ang-1 group (P <0.05). The Ad-Ang-1-transfected group had a significant increase in migration and invasion compared with that of the mock-transfected and Ad-GFP groups The mRNA and protein expression of integrin β1, CD44V6, uPA and MMP-2 in the Ad-Ang-1 group was higher than that in the Ad-GFP and blank control groups (P <0.05) .Compared with mock-transfected and Ad -GFP groups, integrinβ1 and CD44V6 expression intensity greatly increased (P <0.05). CONCLUSION: Transfection of Ang-1 into human gastric cancer cell line BGC-823 can significantly increase expression of integrinβ1 and CD44V6, by which cell adhesion and metastasis to the ECM are promoted.