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目的:分析2010年1月—2014年12月12岁以下儿童流感病毒核酸检测结果,掌握黄南州近年来儿童流感的主要流行株及变化规律,为制定防控措施提供科学依据。方法:收集哨点医院12岁以下流感样病例的咽拭子标本,采用Real-time PCR方法进行核酸检测,对数据采用描述流行病学方法进行分析。结果:共检测1 400份标本,各型流感病毒核酸检测总阳性率为9.1%,阳性标本127份,其中新甲型H1N1流感病毒核酸阳性13份,占10.2%;季节性甲3亚型(H_3N_2)流感病毒核酸阳性48份,占37.8%;季节性乙型流感病毒核酸阳性66份,占52.0%。各年龄组阳性率无明显差异(P>0.05)。结论:黄南州12岁以下儿童中,流感病毒的主要流行株为乙型流感病毒,其次是季节性甲型H_3N_2流感病毒,新甲型H_1N_1流感病毒近年来仍时有检出。
OBJECTIVE: To analyze the detection results of influenza virus nucleic acid in children under 12 years of age from January 2010 to December 2014, master the prevalence of influenza in children in Huangnan Prefecture in recent years, and provide a scientific basis for the development of prevention and control measures. Methods: Throat swab samples of influenza-like illness in sentinel hospitals aged below 12 were collected. Real-time PCR was used to detect nucleic acid. The data were analyzed by descriptive epidemiological method. Results: A total of 1 400 samples were tested. The positive rates of nucleic acid detection for all types of influenza viruses were 9.1% and 127 positive samples respectively, of which 13 were positive for the new influenza A (H1N1) virus, accounting for 10.2% H_3N_2) influenza virus nucleic acid positive 48, accounting for 37.8%; seasonal influenza B virus nucleic acid positive 66, accounting for 52.0%. The positive rate of each age group had no significant difference (P> 0.05). Conclusion: Among the children under 12 years of age in Huangnan Prefecture, the main epidemic strain of influenza virus is Influenza A virus, followed by the seasonal Influenza A H_3N_2 virus, and the influenza A (H1N1) virus is still detected in recent years.