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目的探讨Ｔ辅助细胞（Ｔｈ）亚群功能失衡在婴幼儿哮喘发病中的作用及其影响因素。方法酶联免疫吸附试验方法，对２０例哮喘患儿和１５例健康对照者外周血单个核细胞（ＰＢＭＣ）分别经植物血凝素（ＰＨＡ）和脂多糖（ＬＰＳ）刺激后，培养上清液中各细胞因子含量进行测定。结果经ＰＨＡ刺激后哮喘组Ｔｈ产生ＩＦＮγ、ＩＬ２水平明显低于正常对照组（ｔ′＝４．１５，４．０７；Ｐ均＜０．０１），而ＩＬ４、ＩＬ６、ＩＬ１０水平则显著升高（ｔ′＝４．７３，５．９１，３１８，Ｐ均＜０．０１）。经ＬＰＳ刺激后，哮喘组单核巨噬细胞产生ＩＬ１０水平明显高于正常对照组（ｔ′＝５．６０，Ｐ＜０．０１）而ＩＬ１２水平则降低（ｔ′＝３．３４，Ｐ＜０．０１）。相关分析发现ＩＦＮγ与血清ＩｇＥ水平呈高度负相关（ｒ＝－０．６６４，Ｐ＜０．０１），ＩＬ４、ＩＬ１０与ＩｇＥ呈高度正相关（ｒ＝０．７７６，０７４０；Ｐ＜０．０１）。结论哮喘患儿生成Ｔｈ１类细胞因子不足，Ｔｈ２类因子增多；单核巨噬细胞产生ＩＬ１０增多，ＩＬ１２减少，导致Ｔｈ１／Ｔｈ２功能失衡
Objective To investigate the role of imbalanced T helper (Th) subsets in the pathogenesis of asthma in infants and their influencing factors. Methods The peripheral blood mononuclear cells (PBMCs) of 20 asthmatic children and 15 healthy controls were stimulated by PHA and LPS respectively after the method of enzyme-linked immunosorbent assay (ELISA) In each cytokine content determination. Results After stimulation with PHA, the levels of IFN-γ and IL-2 in asthma group were significantly lower than those in the normal control group (t ’= 4.15,4.07; P <0.01), while IL4 and IL 6, IL-10 levels were significantly increased (t ’= 4.73,5.91,3.18, P <0.01). After stimulated by LPS, IL-10 levels in mononuclear macrophages of asthma group were significantly higher than those in the normal control group (t ’= 5.60, P <0.01) and levels of IL-12 decreased (t’ = 3). 34, P <0.01). Correlation analysis found that IFN γ and serum IgE levels were highly negatively correlated (r = -0.664, P <0.01), IL 4, IL 10 and IgE was highly positively correlated (r = 0.776,0 7740; P <0.01). Conclusions Th1 type cytokines and Th2 type cytokines are not found in children with asthma. The number of IL-10 increased in mononuclear macrophages and IL-12 decreased, resulting in imbalance of Th1 / Th2 function