论文部分内容阅读
ＭＳＵ－１．１细胞株是由ｖ－ｍｙｃ癌基因转染正常二倍体人成纤维细胞后所建立的生命期无限的近二倍体细胞株，使该细胞暴露于实验研究用广谱诱变剂ＩＣＲ－１９１以建立体外细胞转化体系，结果表明剂量０．３５及０．４５μｍｏｌ／Ｌ的ＩＣＲ－１９１可使ＭＳＵ－１．１细胞转化成具有转化灶形成能力和成瘤性的恶性转化细胞。本文支持永生化是人类细胞培养的多步骤的恶性转化过程的前提条件的假说。为建立的致癌物诱发细胞转化体系为研究阐明人类多步致癌的分子机理提供了一个有用的模型体系。
MSU-1.1 cell line is a near-diploid cell line established by transfecting normal diploid human fibroblasts with the v-myc oncogene, exposing the cells to an experimental study using a broad-spectrum mutagenesis ICR-191 was modified to establish an in vitro cell transformation system. The results showed that doses of 0.35 and 0.45 μmol/L of ICR-191 could transform MSU-1.1 cells into malignant transformation with transforming foci formation and tumorigenicity. cell. This article supports the hypothesis that immortalization is a prerequisite for the multi-step malignant transformation of human cell culture. The establishment of a carcinogen-induced cell transformation system provides a useful model system for studying the molecular mechanism of human multi-step carcinogenesis.