论文部分内容阅读
应用细胞培养技术，ＮｏｒｔｈｅｒｎＢｌｏｔ和图像细胞分析技术（ＩＣＭ）研究钙对中国人乳癌细胞株ＢＣａＰ－３７的生长、增殖和基因表达的影响。生长曲线结果显示，钙（１８．０ｍｍｏｌ／Ｌ，３６．０ｍｍｏｌ／Ｌ）对细胞生长具有显著抑制作用（ｐ＜０．０１）。基因表达结果显示，钙（１２．０ｍｍｏｌ／Ｌ）对癌相关基因ＥＧＦＲｍＲＮＡ的表达有抑制作用。测定ＩＣＭ的四项指标包括ＤＩ值，Ｓ期百分比，总ＤＮＡ含量及核面积。结果显示，两实验组（１．２ｍｍｏｌ／Ｌ，１２．０ｍｍｏｌ／Ｌ）ＤＩ值分别为１．７８和１．７７，表现为恶性细胞的特征。其它三项指标均显著低于对照组（ｐ＜０．０１，ｐ＜０．０５）。综合评价ＩＣＭ各项指标结果显示，钙对细胞的增殖具有显著抑制作用。
The effects of calcium on the growth, proliferation and gene expression of Chinese breast cancer cell line BCaP-37 were studied by using cell culture technique, Northern Blot and image cell analysis (ICM). Growth curve results showed that calcium (18.0 mmol/L, 36.0 mmol/L) had a significant inhibitory effect on cell growth (p<0.01). The results of gene expression showed that calcium (12.0 mmol/L) inhibited the expression of cancer associated gene EGFR mRNA. The four indicators for determining ICM included DI, S-phase percentage, total DNA content, and nuclear area. The results showed that the DI values of the two experimental groups (1.2 mmol/L, 12.0 mmol/L) were 1.78 and 1.77, respectively, which were characteristic of malignant cells. The other three indicators were significantly lower than the control group (p<0.01, p<0.05). Comprehensive evaluation of ICM indicators showed that calcium has a significant inhibitory effect on cell proliferation.