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采用小麦小穗离体培养法 ,研究了在含蔗糖和琼脂的MS培养基中添加BR12 0 或BR对颖果形成的影响。在开花前 4d小穗培养中 ,添加 0 .0 10、0 .10 0mg/LBR12 0 或 0 .0 1mg/LBR可使小穗颖果数由对照的 1.4粒增加至 2 .1～ 2 .5粒 ,而颖果重比对照提高 31%～ 84 % ;在开花后 2d的小穗培养中 ,则可使颖果数由对照的 1.9粒增至 2 .5～ 3.0粒 ,其颖果重提高 34 %～ 50 %。表明BR12 0 和BR可能通过改善受精过程 ,促进受精子房发育及防止其退化 ,而促进颖果的形成。同时证明经BR12 0 于母株上前处理的小穗 ,在培养中也表现良好促进颖果形成效果。
Using wheat spikelet in vitro culture, the effects of BR12 0 or BR on MS medium containing sucrose and agar were studied. In spikelet culture at 4 days before flowering, the number of spikelets increased from 1.4 to 2.21 to 2.5 after the spikelet culture was supplemented with 0.010, 0.010 mg / L BR12 0 or 0.100 mg / L BR While the caryopsis weight increased by 31% ~ 84% compared with the control. In the spikelet culture at 2 days after flowering, the number of caryopsis increased from 1.9 to 2.5 to 3.0 in the control, and the caryopsis weight increased 34% ~ 50%. This suggests that BR12 0 and BR may promote the formation of caryopsis by improving the process of fertilization, promoting the development of the fertilized sperm and preventing its degeneration. At the same time, it was demonstrated that the spikelets pretreated with BR12 0 in the mother plant also showed good effect in promoting caryopsis formation in culture.