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移植物抗宿主病(GVHD)和肿瘤复发限制了同种异体或自体骨髓移植(BMT)的应用,输注除去淋巴细胞(参与GVHD)或肿瘤细胞的造血多能干细胞的浓缩液可以改善骨髓移植的预后。作者应用非连续的蛋白梯度法从2个BMT的供体和25个正常的供髓者,一个多发性骨髓瘤(MM)和一个有骨髓浸润的小细胞性肺癌(SCCL)的病人中,将骨髓细胞分离为4个部份;CFUc,TCFUc和PHA反应部份。最佳的分离条件是在密度平衡之前,于离心30分钟后,停止离心,可获得300mM的骨髓液。在含有10％骨髓细胞的低密度第3部份中CFUc浓度增加了9倍。应用HLA完全相同的富含CFUc对PHA不起反应的骨髓
Graft versus host disease (GVHD) and tumor recurrence limit the use of allogeneic or autologous bone marrow transplantation (BMT). Infusion of concentrates of hematopotensive pluripotent stem cells that remove lymphocytes (involved in GVHD) or tumor cells may improve bone marrow transplantation The prognosis. Using a non-continuous gradient of protein from patients with two BMTs and 25 normal donors, one with multiple myeloma (MM) and one with small cell lung cancer (SCCL) infiltrating the bone marrow, Bone marrow cells were separated into 4 parts; CFUc, TCFUc and PHA reaction parts. The best separation conditions are centrifugation for 30 minutes before centrifugation is stopped and 300 mM bone marrow fluid is available. The CFUc concentration increased 9-fold in the low-density part 3 containing 10% bone marrow cells. HLA-identical bone marrow rich in CFUc to PHA is used