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本文采用离体血管收缩功能实验方法，比较12月与10周龄大鼠主动脉α1肾上腺素受体（α1－AR）及其亚型的分布。结果显示：12月龄大鼠与10周龄大鼠相比，去甲肾上腺素（NE）引起的最大收缩反应显著降低，而半效激动浓度（pD2）值无显著性改变；氯乙基可乐定（CEC，不可逆阻断。α1B－AR和α1D－AR亚型）对NE引起血管收缩的抑制作用明显减弱；WB4101（α1A－AR和α1D－AR选择性拮抗剂）抬抗NE缩血管效应的pA2值没有改变；BMY7378（α1D－AR选择性拮抗剂）的半效抑制浓度（pA2）值明显降低；Sertindole（α1A－AR选择性拮抗剂）的pA2值显著增大。上述结果提示，12月龄大鼠介导主动脉收缩效应的α1－AR由10周龄以。α1D－AR亚型为主转变为α1A－AR和α1D－AR亚型共同参与，且以α1A－AR亚型为主。
In this study, we used the ex vivo vasoconstriction test to compare the distribution of α1-adrenergic receptor (α1-AR) and its subtypes in the aorta of rats aged 12 and 10 weeks. The results showed that the maximal contractile response to norepinephrine (NE) was significantly reduced in 12-month-old rats compared with 10-week-old rats, while the pD2 did not change significantly in the 12-month-old rats. (CEC), irreversible blockade of α1B-AR and α1D-AR subtypes significantly inhibited NE-induced vasoconstriction; WB4101 (α1A-AR and α1D-AR selective antagonists) pA2 did not change; the half-inhibitory concentration (pA2) value of BMY7378 (α1D-AR selective antagonist) was significantly decreased; and the pA2 value of Sertindole (α1A-AR selective antagonist) was significantly increased. The above results suggest that α1-AR in 12-month-old rats mediates aortic contractile effects by 10 weeks of age. α1D-AR subtypes mainly changed into α1A-AR and α1D-AR subtypes to participate in, and the main α1A-AR subtype.