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研究了铅对出生后7～21 d大鼠海马的损伤以及应用维生素C后的影响,探讨了维生素C的保护作用.将健康1周龄Wistar大鼠随机分为铅染毒组,维生素C给药组及生理盐水组.铅染毒组、维生素C给药组每组各18只,生理盐水组每组6只,共42只.铅染毒组生后6 d起每天7.5 mL·kg~(-1)腹腔注射浓度0.05%的醋酸铅;维生素C给药组出生后6 d起每天按相同剂量醋酸铅注射的同时5 mL·kg~(-1)(125 g·L~(-1))维生素C灌胃;生理盐水组生后6日起每天腹腔注射7.5 mL·kg~(-1)灭菌生理盐水.各组于出生后第2、3、4周取材.测量血铅,分别进行Bcl-2、Bax免疫组织化学染色及Fluoro-Jade B荧光染色,并作平均光密度分析.通过对各个时间段每组血铅测定,可得维生素C给药组血铅含量为(2.395±0.974)μmol·L~(-1)低于铅染毒组(3.164±1.481)μmol·L~(-1).Bcl-2铅染毒组海马区阳性细胞表达少于给药组(P<0.01),而Bax多于给药组(P<0.01),Flouro-JadeB铅染毒组海马区阳性细胞率明显多于给药组(P<0.01).此结果提示维生素C可降低染铅幼年大鼠的血铅浓度,并具有一定抗海马细胞损伤作用.
The effects of lead on the hippocampus of rats 7 ~ 21 days after birth and the effect of vitamin C on the protective effect of vitamin C were studied.A healthy one-week-old Wistar rats were randomly divided into lead-exposed group, vitamin C Drug group and saline group.The lead poisoning group and vitamin C group were given 18 rats in each group and the saline group were 6 rats in each group.There were 42 rats in each group.Drug poisoning group was 7.5 mL · kg ~ (-1) were intraperitoneally injected with 0.05% lead acetate. Vitamin C administration group was injected with 5 mL · kg -1 (125 g · L -1) of lead acetate at the same dose every day for 6 d after birth, Vitamin C was administrated intraperitoneally with saline (7.5 mL · kg -1) at 6 days after birth, and blood samples were collected at 2, 3 and 4 weeks after birth respectively Bcl-2, Bax immunohistochemical staining and Fluoro-Jade B fluorescent staining, and the average optical density analysis.According to the determination of blood lead in each time period, the blood lead content in vitamin C group was (2.395 ± 0.974 ) μmol·L -1 was lower than that of the lead-exposed group (3.164 ± 1.481 μmol·L -1) .The positive cells in hippocampus of Bcl-2-exposed group were less than that of the control group (P <0.01) ), While Bax was more than that of the administration group (P <0.01), Flouro -JadeB lead-exposed group hippocampus significantly higher than the rate of positive cells (P <0.01) .This result suggests that vitamin C can reduce blood lead levels in lead-poisoning young rats, and have a certain anti-hippocampal cell injury.