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用妊娠第7d大鼠,实验Ⅰ和Ⅱ组分别每日灌服硝酸铅100mg/kg和200mg/kg,连续5d。于妊娠第12d检查,实验Ⅰ组血铅浓度27.90±8.47μg/dl,黄体和胎盘可出现轻度损害。实验Ⅱ组孕鼠血铅浓度48.77±16.15μg/dl,相当于人亚中毒血铅水平,黄体和胎盘的光镜或超微结构损害严重,黄体3β-羟甾脱氢酶为对照组的1/20,31.69％的胚胎被吸收。垂体前叶细胞未见明显变化。人胎盘绒毛体外培养,实验组的培养液内分别加入硝酸铅0.5μg/ml、2.5μg/ml、5μg/ml,培养3～4d后进行形态学观察。培养液中含铅量2.5μg/ml以上者,绒毛的光镜及超微结构均有明显损害,培养液中hCG含量也明显减少。本文结果提示,铅使孕鼠的黄体、胎盘及胎鼠毒性损害明显,对人胎盘绒毛也有直接毒害作用。
Rats in the 7th day of gestation, groups I and II were fed with 100 mg/kg lead nitrate and 200 mg/kg lead for 5 days. On the 12th day of pregnancy, the blood lead concentration in the experimental group I was 27.90±8.47 μg/dl, and slight damage was observed in the corpus luteum and placenta. The blood lead concentration of pregnant rats in experimental group II was 48.77±16.15μg/dl, which was equivalent to the blood lead level of human sub-toxicity. The light microscope or ultrastructural damage of the corpus luteum and placenta was severe, and the corpus luteum 3β-hydroxysteroid dehydrogenase was the control group. /20, 31.69% of embryos were absorbed. There was no significant change in the anterior pituitary cells. Human placental villus was cultured in vitro. Lead nitrate 0.5μg/ml, 2.5μg/ml, and 5μg/ml was added to the culture fluid of the experimental group, and morphological observation was performed after 3 to 4 days of culture. When the amount of lead in culture medium was more than 2.5 μg/ml, the light microscope and ultrastructure of villus were significantly damaged, and the hCG content in culture medium was also significantly reduced. The results of this article suggest that lead makes pregnant rats’ corpus luteum, placenta and fetal rat toxicity damage, but also has a direct toxic effect on human placental villi.