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在食用菌组织分离培养中,一股资料介绍均为采集子实体后立即进行组织接种分离,这对人工栽培材料来说是容易作到的,但是分离野生材料时,就比较困难了。由于野外现场无菌条件差,不少好的野生材料往往因污染未能分离成功。为了解决这一问题,我们进行了以蘑菇(Agaricus bisporus)为试材的组织分离菌丝生活力时间的试验。 (一)方法:将采集的人工栽培蘑菇,放置在经(8g高锰酸钾/m~3+16ml福尔马林/m~3)熏蒸消毒过的接种箱内,临接种前用烧杯盖好试验材料后,打开箱内紫外灯照射30分钟。然后将蘑菇子实体用经75％酒精消毒过的
In the separation and cultivation of edible fungi, an information introduction is to collect the fruiting body immediately after tissue isolation, which is easy to do for the artificial cultivation of materials, but the separation of wild materials, it is more difficult. Due to the poor aseptic conditions in the field, many good wild materials are often not successfully separated due to contamination. In order to solve this problem, we carried out the experiment of separating mycelium viability from the tissue of Agaricus bisporus. (A) Methods: The collected artificial cultivation mushroom placed in the (8g potassium permanganate / m ~ 3 + 16ml formalin / m ~ 3) fumigation vaccination box, After a good test material, open the box UV lamp irradiation for 30 minutes. The mushroom fruiting body is then sterilized with 75% alcohol