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目的 探讨癌基因相关蛋白p2 1,p185及增殖细胞核抗原 (PCNA)在大肠癌癌旁移行粘膜、近端断端大肠粘膜、腺瘤型息肉中表达的意义及其与大肠癌的关系。方法 应用免疫组织化学方法对 40例原发性大肠癌及癌旁移行粘膜 ,2 0例腺瘤型息肉中p2 1,p185及PCNA的表达进行检测。结果 p2 1,p185及PCNA在大肠癌癌旁移行粘膜中的阳性表达率分别为 2 2 5 % (9 40 ) ,17 5 % (6 40 ) ,10 0 % (4 0 40 ) ;p2 1,p185的阳性表达与临床病理分期无关 ;p2 1,p185 ,PCNA表达分级在癌组织及癌旁移行粘膜中存在正相关。结论 在癌旁移行粘膜中p2 1,p185表达异常、细胞增殖力学异常 ,反映癌旁移行粘膜是一种不稳定的癌前状态 ,可能与大肠癌复发有一定关系
Objective To investigate the significance of oncogene-related proteins p21, p185 and proliferating cell nuclear antigen (PCNA) expression in colorectal mucosa, proximal end colorectal mucosa and adenoma polyp in colorectal cancer and its relationship with colorectal cancer. Methods Immunohistochemistry was used to detect the expression of p21, p185 and PCNA in 40 cases of primary colorectal cancer, adjacent non-cancerous mucosa and 20 cases of adenomatous polyps. Results The positive expression rates of p21, p185 and PCNA in colorectal mucosa of colorectal cancer were 25% (95%), 17 5% (640), 100% (40% The positive expression of p185 was not related to the clinicopathological stage. There was a positive correlation between the expression of p21, p185 and PCNA in the cancerous tissue and adjacent mucosa. Conclusions The abnormal expression of p21 and p185 in the mucosa adjacent to the cancer and the abnormal cell proliferation mechanics may reflect an unstable precancerous mucosa and may be related to the recurrence of colorectal cancer