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血浆中CO 经由RBC 携带并由连二亚硫酸盐还原法测定。批内和批间CV 分别为10.7%和12.8%。最低检出限为0.1mg/L。血浆中CO 的正常参考范围为0.14～0.60mg/L。此法简便、灵敏。在空气中CO 持续浓度>4ml/L 对人有致命性。CO 与Hb 的结合力比O_2与Hb 的结合力大200倍还多,HbCO 使得O_2的运输无效,人体RBC 中HbCO浓度占总Hb 浓度的65～80%通常使人致死,浓度较低的导致不可逆转的神经后遗症。经典观点认为与Hb 结合的CO 夺取了组织中的氧,引起细胞的死亡。而有人却认为CO 通过与细胞色素a_3的结合,抑制了氧化磷酸盐化作用和组织利用氧的能力,致死细胞。
CO in plasma is carried via RBC and determined by the dithionite reduction method. The intra-and inter-CVs were 10.7% and 12.8% respectively. The minimum detection limit of 0.1mg / L. The normal reference range for CO in plasma is 0.14 to 0.60 mg / L. This method is simple and sensitive. Continued concentration of CO in the air> 4ml / L is fatal to humans. The binding force between CO and Hb is more than 200 times larger than the binding force between O_2 and Hb. HbCO makes O_2 transport ineffective. HbCO concentration in human RBC accounts for 65-80% of the total Hb concentration, which usually leads to death and low concentration Irreversible neurological sequelae. The classical view is that CO bound to Hb sequesters oxygen in tissues and causes cell death. However, some people think that CO binds to cytochrome a_3 and inhibits oxidative phosphorylation and the ability of tissues to use oxygen to kill cells.