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第Ⅰ报报道,用定性组织化学方法,发现人参的活性成分人参皂甙分布于人参根的周皮和皮层中,木质部和髓部未含存。本文对人参根各组织进行人参皂甙含量的定位测定。材料取自日本长野县栽培的一年生至六年生人参植株,将植株按各部位分开,干燥、粉碎,供分析用。方法:(1)人参皂甙元的气相定量分析按Tanaka法,把脱脂人参粉末的甲醇提取物用5%硫酸——乙醇(3:1)水解,经沸水浴上加热6小时,水解产物的醚溶性甙元部分用碱性水分离,除去齐墩果酸。残余物主要含人参二醇(从人参皂甙Rb、Rc类衍生)和人参二醇(从人参皂甙Rg类衍生),经N-三甲基硅烷(TMS)化室温处理一小时,TMS产物进行气相层析分析。仪器:岛津气相层
The first report reported that using qualitative histochemical methods, it was found that ginsenosides, the active ingredient of ginseng, were distributed in the periderm and cortex of ginseng roots, and xylem and pith were not present. In this paper, the determination of ginsenosides content in ginseng root tissue was performed. The materials were taken from annual to six-year-old ginseng plants grown in Nagano Prefecture, Japan. The plants were separated from each site and dried and crushed for analysis. Methods: (1) Gas phase quantitative analysis of ginsenosides According to the method of Tanaka, the methanol extract of defatted ginseng powder was hydrolyzed with 5% sulfuric acid-ethanol (3:1) and heated on a boiling water bath for 6 hours to hydrolyze the ether of the product. The soluble components are partially separated by alkaline water to remove oleanolic acid. The residue consists mainly of panaxadiol (derived from ginsenosides Rb and Rc) and panaxadiol (derived from ginsenoside Rg) and treated with N-trimethylsilane (TMS) at room temperature for one hour. The TMS product is in the gas phase. Chromatography analysis. Instrument: Shimadzu vapor layer