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本发明涉及一种检测克百威农药残留的免疫传感器制备方法,其特征在于:自组装4’4-硫代联苯硫酚、电沉积纳米金,重复第一步和第二步2遍,与传统的方法相比,采用4’4-硫代联苯硫酚两端的巯基以及其刚性结构,巯基一端通过金硫键连接裸金电极,巯基另一端通过金硫键连接电沉积的纳米金,并通过层层自组装的方法,构建了一种具有高稳定性、高度有序的金硫体系。由于纳米金较高的比表面积、稳定性和导电性可以增大电极表面电子的转移和生物相容性;通过层层自组装,扩大了电极的有效固定面积,提高了电极表面抗体的有效固定量,从而使制备的电流型免疫传感器检测时间较短,灵敏度高,选择性稳定性好,再生能力好和回收率符合要求。
The invention relates to a preparation method of an immunosensor for detecting carbofuran pesticide residues, which comprises the following steps: self-assembling 4’4-thiobisthiol, electrodepositing nanogold, repeating first step and second step twice, Compared with the traditional method, the thiol group at both ends of 4’4-thiophenol thiophenol and its rigid structure are adopted. One end of the thiol group is connected to the bare gold electrode through the gold-sulfur bond and the other end of the thiol group is connected with the electrodeposited gold nano- , And through the method of multi-layer self-assembly, a highly stable and highly ordered gold-sulfur system was constructed. Due to the high specific surface area, stability and conductivity of nano-gold, the surface electron transfer and biocompatibility of the electrode can be increased. By layer-by-layer self-assembly, the effective fixed area of the electrode is enlarged and the effective immobilization of the antibody on the surface of the electrode is enhanced So that the preparation of the current-type immunosensor detection time is short, high sensitivity, good selectivity, good regeneration and recovery meet the requirements.