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目的 :利用 KCl滴注大脑皮质制作大鼠皮层传播性抑制 (cortical spreading depression,CSD)模型 ,研究94- 95 - 10 L对 c- fos基因在大鼠脑额顶区内扣带回、新皮质和梨状区表达的影响。方法 :雄性 Wistar大鼠麻醉后 ,在左侧脑表面滴注 KCl(1mol/ L ,5μl)之前 5 0 m in,给予 94- 95 - 10 L (30 mg/ kg,ip.) ,氟桂嗪 (3m g/ kg,iv.)作为阳性对照。用免疫组化法观察 c- fos阳性细胞在以上 3区的表达。结果 :左侧脑表面滴注 KCl 12 0 min后 ,各区的 c- fos表达阳性细胞明显增多 ,而对侧基本无表达 ;尾静脉注射氟桂嗪能明显抑制 c- fos的表达 ;腹腔注射 94- 95 - 10 L使 c-fos表达明显减少。结论 :在由 KCl制造的 CSD模型中 ,94- 95 - 10 L能明显抑制 c- fos在额顶区的表达 ,说明该药对大鼠 CSD模型引起的偏头痛先兆有抑制作用
OBJECTIVE: To study the effects of 94-95-10 L on the c-fos gene c-fos gene in the frontal cortex of rats by using KCl instillation of the cerebral cortex in a rat model of cortical spreading depression (CSD) And pear-shaped area of expression. METHODS: Male Wistar rats were anesthetized and infused with 94-95 - 10 L (30 mg / kg, ip.) Of KCl (1mol / L, 5μl) (3 m g / kg, iv.) As a positive control. Immunohistochemistry was used to observe the expression of c-fos positive cells in the above three regions. Results: After the KCl 12 min on the left side of the brain, the expression of c-fos positive cells in each area increased significantly, while the contralateral side showed almost no expression. The injection of flunarizine into the caudal vein significantly inhibited the expression of c-fos. - 95 - 10 L significantly reduced c-fos expression. CONCLUSIONS: In the CSD model made by KCl, 94-95 - 10 L significantly inhibited the expression of c-fos in the frontal area, indicating that this drug has an inhibitory effect on migraine aura by CSD model in rats