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目的 :评价国产乙基纤维素微球的安全性及其在口腔颌面部动脉的栓塞效果。方法 :采用体外细胞培养技术 ,以 MTT法检测乙基纤维素微球的细胞生物学效应 ;在 X线监控下 ,将乙基纤维素微球选择栓塞在犬颈外动脉及其分支 ,在栓塞后的即刻和一月后测量其颈部各主要动脉的血液流速及流量 ,进行统计分析。不同时间取栓塞组织石蜡切片 ,常规 HE染色 ,光镜观察。结果 :细胞学实验表明 ,国产乙基纤维素微球的浸提液不影响细胞生长和增殖。动物实验表明 ,被栓塞血管的流量、流速明显降低 ,基本不形成侧枝循环 ,临近动脉的血流量则代偿性的增加。组织学观察可以见到微球成簇状堵塞末梢动脉血管 ,达到较为完全的栓塞效果。结论 :国产乙基纤维素微球生物相容性良好 ,可以持久阻塞动脉血管腔 ,有望在临床上用于血管性疾病的栓塞治疗。
Objective: To evaluate the safety of domestic ethyl cellulose microspheres and their embolization in oral and maxillofacial arteries. METHODS: The cell biological effects of ethyl cellulose microspheres were detected by MTT assay in vitro. Under the monitoring of X-rays, the ethyl cellulose microspheres were embolized in the external carotid artery and its branches in the embolization. Immediately and after one month later, the blood flow rate and flow rate of the major arteries of the neck were measured and statistical analysis was performed. Embolized tissue paraffin sections at different times, routine HE staining, and light microscopy. Results : Cytological experiments showed that the extracts of domestic ethyl cellulose microspheres did not affect cell growth and proliferation. Animal experiments showed that the flow rate and flow rate of the embolized blood vessels were significantly reduced, and collateral circulation was almost not formed, and the blood flow of adjacent arteries was compensatory increase. Histological observation can see that the microspheres clustered and blocked the peripheral arterial blood vessels to achieve a more complete embolization effect. Conclusion: The domestic ethylcellulose microspheres have good biocompatibility and can permanently block the arterial lumen. It is expected to be used clinically for embolization of vascular diseases.