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目的:探讨乙型肝炎患者病毒核酸载量与血清乙型肝炎病毒外膜大蛋白(HBV-LP)含量的关系。方法:采用酶联免疫吸附试验(ELISA)和实时荧光定量PCR法分别检测307例乙型肝炎患者血清的HBV-LP、HBV-M和HBVDNA,并进行相关性分析。结果:307例乙型肝炎患者血清中HBV-LP含量与HBV DNA拷贝数变化相一致,两者呈正相关(r=0.943);在131份HBeAg阳性血清中,HBV DNA与HBV-LP的阳性率分别为95.42%和93.89%,两者差异无显著性(P>0.05);在176份HBeAg阴性血清中,HBV DNA与HBV-LP的阳性率分别为46.02%和56.25%,两者差异无显著性(P>0.05)。结论:血清HBV-LP水平能反映HBV感染者体内HBV复制程度,其灵敏度高于HBeAg,可作为判断HBV复制新的血清学指标。
Objective: To investigate the relationship between viral nucleic acid load of hepatitis B patients and the content of hepatitis B virus outer membrane large protein (HBV-LP) in patients with hepatitis B virus. Methods: The serum levels of HBV-LP, HBV-M and HBVDNA in 307 hepatitis B patients were detected by enzyme-linked immunosorbent assay (ELISA) and real-time fluorescence quantitative PCR respectively. Results: The serum levels of HBV-LP in 307 hepatitis B patients were consistent with those of HBV DNA copy number (r = 0.943). The positive rates of HBV DNA and HBV-LP in 131 HBeAg-positive sera (95.42%) and 93.89% (93.89%) respectively. There was no significant difference between the two groups (P> 0.05). The positive rates of HBV DNA and HBV-LP in 176 HBeAg negative sera were 46.02% and 56.25% (P> 0.05). Conclusion: The level of serum HBV-LP can reflect the level of HBV replication in HBV-infected patients, and its sensitivity is higher than that of HBeAg, which can be used as a new serological marker for HBV replication.