Chemical synthesis of Ub-AMC via ligation of peptide hydrazides

• 作 者: LIANG Jun FANG GeMin HUANG Xiu
• 机 构: HighMagneticFieldLaboratory,UniversityofScienceandTechnologyofChina,MinistryofEducationProteinScienc
• 出 处: 中国科学：化学英文版
• 发表日期: 2013年9期
• 关键词: 化学合成 合成肽 结扎 酰肼 甲基香豆素 生物学功能 泛素化 活性分析 h the measurement of its DUB activity.ligati
• 基金项目: National Basic Research Program of China （973 program, 2013CB932800）, the National Natural Science Foundation of China （NSFC, 31100524 to M.Z., 31170817 for C.T., and 20972148 to L.L.）

The C-terminal conjugate of ubiquitin with 7-amino-4-methylcoumarin (Ub-AMC) is an important probe for fluorescencebased analysis of deubiquitinating enzyme (DUB) activity. It is important to develop more efficient methods for the preparation of Ub-AMC be